HPLC Service & Repair

HPLC separates compounds by pumping a liquid mobile phase through a column under pressure, and the pump, autosampler, and column all wear out at rates directly proportional to run volume. ILS identifies which component is actually driving a symptom before replacing anything, so a worn seal doesn’t get mistaken for a failing column.

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How ILS Diagnoses and Resolves Common HPLC Issues

HPLC has only a few real points of failure, but pump, valve, and column wear all produce nearly the same symptoms: a pressure shift, a retention time drift, a change in peak shape.

Pump Seal and Check Valve Repair

Pump seals and check valves wear under repeated pressure cycling, showing up as pressure fluctuation before the pump fails outright.

ILS replaces worn seals and valves once pressure trends confirm the pump as the actual cause.

Autosampler Needle and Valve Repair

Autosampler needles and injection valves wear from routine injection cycles, showing up as carryover or retention time drift.

ILS reviews injection history to distinguish autosampler wear from a column or mobile phase issue producing the same symptom.

Column and Detector Troubleshooting

Column performance degrades from repeated sample matrix exposure, showing up as peak tailing or reduced resolution.

ILS separates a degrading column from a detector issue by comparing performance across both, since either can produce the same loss of resolution.

Mobile Phase and Pressure Diagnostics

Inconsistent mobile phase handling accelerates wear across the pump, valves, and column alike, and a delivery issue often gets attributed to whichever component shows the symptom first.

ILS traces pressure and flow irregularities back to the delivery system when that’s the actual source, not the downstream component that happened to show it.

Our HPLC Service Capabilities

Separate the Interface Points

Our engineers compare pressure, injection, and resolution data across the pump, valves, and column.

Train Across Platforms

Our engineers train across both Agilent and Shimadzu HPLC platforms.

Schedule Around Usage

We set the visit frequency based on real run volume and instrument usage.

Confirm Pressure Post-Repair

We confirm system pressure and peak resolution are back within factory tolerance after every repair.

HPLC Systems We Service

Agilent and Shimadzu HPLC systems are used in pharmaceutical, biotech, environmental, and food testing labs, supporting everything from routine separations and method development to QC testing and impurity profiling. The two often run side by side in the same lab, and ILS covers both under one contract.